Sample (50 μg of HeLa whole cell lysate)
GTX100911 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin B1 antibody (GTX100911) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin B1 stained by Cyclin B1 antibody (GTX100911) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin B1 antibody (GTX100911) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin B1 stained by Cyclin B1 antibody (GTX100911) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Sample (30 ug of whole cell lysate)
A: U2OS
B: U2OS treated with 100ng/ml Nocodazole for 16hr
10% SDS PAGE
GTX100911 diluted at 1:1000

Cyclin B1 antibody detects Cyclin B1 protein by Western blot analysis.
A. 20 μg 293T whole cell lysate/extract
B. 10 μg 293T whole cell lysate/extract
C. 5 μg 293T whole cell lysate/extract |
D. 1 μg 293T whole cell lysate/extract
10 % SDS-PAGE
Cyclin B1 antibody (GTX100911) dilution: 1:3000

Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
Cyclin B1 antibody (GTX100911) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal PLoS One in 2014. PMID: 25105411

The data was published in the journal J Biomed Sci in 2012. PMID: 22283874

The data was published in the journal World J Surg Oncol in 2018. PMID: 29466986