Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3-2] (GTX110541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Cyclin D1 antibody [N1C3] detects Cyclin D1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX110541) diluted at 1:1000.