Wild-type (WT) and Cyclophilin F knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin F antibody (GTX117951) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin F antibody (GTX117951) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin F antibody (GTX117951) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed A431, using PPIF(GTX117951) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPIF(GTX117951) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin F antibody (GTX117951) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.