Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cytochrome C antibody [HL2205] detects Cytochrome C protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
Cytochrome C stained by Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Cytochrome C antibody [HL2205] detects Cytochrome C protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Cytochrome C stained by Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Cytochrome C antibody [HL2205] detects Cytochrome C protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Cytochrome C stained by Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.