Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Cytokeratin 10 antibody (GTX113221) diluted at 1:10000 and competitor's antibody (sc-15367) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.