Immunofluorescence analysis of methanol-fixed MCF-7, using Cytokeratin 13(GTX110308) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded CAL27 xenograft, using Cytokeratin 13(GTX110308) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 13 antibody (GTX110308) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.