Immunohistochemical analysis of paraffin-embedded Breast ca, using Cytokeratin 2(GTX117806) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX117806 diluted at 1:1000

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 2 antibody (GTX117806) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.