Application Note
Used to identify and differentiate different carcinomas. This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. For optimal results, tissue sections should either be treated with high-temperature based antigenic unmasking technique, or with proteolytic enzymes such as AutoZyme or Pronase. The optimal conditions should be determined by the individual laboratory. This antibody is not suitable for use on cryostat sections or cell smears due to non-specific cross-reaction with negative epithelia. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies.
Calculated MW
Positive Control
Human Normal Colon, Colon Carcinoma
Product Note
This antibody reacts with the 46 kD cytokeratin polypeptide 20, a type I cytokeratin that is found in duodenal epithelium, glandular gastric and pyloric epithelium, urothelium and Merkel cells of the epidermis. It does not cross-react with other cytokeratin polypeptides or other intermediate filaments. This cytokeratin is expressed in colon carcinoma, and to a lesser degree in gastric carcinoma. Other positive carcinomas include gall bladder, bile duct, ductal cells of pancreas, mucinous ovarian tumors and transitional cell carcinomas. Carcinoma of breast, lung, endometrium and non-mucinous tumors of ovary are not recognized byThis antibody. Merkel cell carcinomas of the skin show strong reactivity withThis antibody.
Form
Liquid
Buffer
PBS
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Cytokeratin preparation of villi of human duodenal mucosa.
Conjugation
Unconjugated
RRID
AB_383336
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
keratin 20 , CD20 , CK-20 , CK20 , K20 , KRT21
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
Database
Research Area