Cytokeratin 20 antibody [N2C2], Internal detects Cytokeratin 20 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Cytokeratin 20 stained by Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using Cytokeratin 20(GTX110600) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20(GTX110600) antibody at 1:200 dilution.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 20 antibody [N2C2], Internal (GTX110600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

The data was published in the journal Proteome Sci in 2014. PMID: 25075203