Sample (30 ug of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX112183 diluted at 1:1000

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 23 antibody (GTX112183) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.