Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 33B(GTX112454) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 33B(GTX112454) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX112454 diluted at 1:10000

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 33B antibody (GTX112454) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cytokeratin 33B antibody detects Cytokeratin 33B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog squamous cell carcinoma.
Cytokeratin 33B stained by Cytokeratin 33B antibody (GTX112454) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min