Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 5 antibody (GTX113219) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 5(GTX113219) antibody at 1:200 dilution.

Cytokeratin 5 antibody detects Cytokeratin 5 protein at cell membrane and cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Cytokeratin 5 antibody (GTX113219) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min