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Cytokeratin 8 antibody

KO/KD
Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX32549
Anti-Cytokeratin 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX32549
Anti-Cytokeratin 8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32549
Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX32549

Cat. No. GTX32549

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Cytokeratin 8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-483 of human KRT8 (NP_002264.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 8 , CARD2 , CK-8 , CK8 , CYK8 , K2C8 , K8 , KO

Cellular Localization

Cytoplasm,nucleoplasm

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX32549

GTX32549 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX32549 Cytokeratin 8 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Cytokeratin 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX32549

GTX32549 IHC-P Image

IHC-P analysis of human appendix tissue using GTX32549 Cytokeratin 8 antibody.
Dilution : 1:100

Anti-Cytokeratin 8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32549

GTX32549 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32549 Cytokeratin 8 antibody.
Blue : DAPI
Dilution : 1:100

Anti-Cytokeratin 8 antibody used in Western Blot (WB). GTX32549

GTX32549 WB Image

WB analysis of various sample lysates using GTX32549 Cytokeratin 8 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399