DDB1 antibody

Name: DDB1 antibody
Brand: GeneTex
SKU: GTX100130
Price: 399.00 USD

Anti-DDB1 antibody used in Western Blot (WB). GTX100130

Anti-DDB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100130

Anti-DDB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100130

Cat. No. GTX100130

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all DDB1 products

View product citations for antibody GTX100130 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:500-1:1000

Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

NIH-3T3 , Mouse brain , PC-12 , Rat2 , rat brain , 293T

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DDB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950102

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

damage specific DNA binding protein 1 , DDBA , UV-DDB1 , XAP1 , XPCE , XPE , XPE-BF

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. [provided by RefSeq]

Database

Gene ID: 1642 DDB1
UniProt: Q16531 DDB1

Research Area

DATA IMAGES

GTX100130 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody (GTX100130) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100130 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using DDB1(GTX100130) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100130 IHC-P Image

DDB1 antibody detects DDB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
DDB1 stained by DDB1 antibody (GTX100130) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100130 IHC-P Image

DDB1 antibody detects DDB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
DDB1 stained by DDB1 antibody (GTX100130) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100130 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDB1(GTX100130) antibody at 1:200 dilution.

GTX100130 IHC-P Image

DDB1 antibody detects DDB1 protein at nucleus and cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
DDB1 antibody (GTX100130) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100130 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody (GTX100130) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100130 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody (GTX100130) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100130 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX100130 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100130 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX100130 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.