Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DDR1 antibody [N1N3] (GTX111453) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DDR1 antibody [N1N3] (GTX111453) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DDR1 antibody [N1N3] detects DDR1 protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
DDR1 stained by DDR1 antibody [N1N3] (GTX111453) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using DDR1(GTX111453) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX111453 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.