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DDR2 antibody [HL1107]

Recombinant antibody KO/KD Overexpression
Anti-DDR2 antibody [HL1107] used in Western Blot (WB). GTX636322
Anti-DDR2 antibody [HL1107] used in Western Blot (WB). GTX636322
Anti-DDR2 antibody [HL1107] used in IHC (Paraffin sections) (IHC-P). GTX636322
Anti-DDR2 antibody [HL1107] used in Western Blot (WB). GTX636322
Anti-DDR2 antibody [HL1107] used in IHC (Paraffin sections) (IHC-P). GTX636322
Anti-DDR2 antibody [HL1107] used in IHC (Paraffin sections) (IHC-P). GTX636322
Anti-DDR2 antibody [HL1107] used in Western Blot (WB). GTX636322

Cat. No. GTX636322

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1107

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DDR2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

HeLa

Product Note

This antibody was raised against human DDR2 Intracellular domain.

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human DDR2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909967

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

discoidin domain receptor tyrosine kinase 2 , MIG20a , NTRKR3 , TKT , TYRO10 , WRCN

Cellular Localization

Cell membrane

Background

This gene encodes a member of the discoidin domain receptor subclass of the receptor tyrosine kinase (RTKs) protein family. RTKs play a key role in the communication of cells with their microenvironment. The encoded protein is a collagen-induced receptor that activates signal transduction pathways involved in cell adhesion, proliferation, and extracellular matrix remodeling. This protein is expressed in numerous cell types and may alos be involved in wound repair and regulate tumor growth and invasiveness. Mutations in this gene are the cause of short limb-hand type spondylometaepiphyseal dysplasia. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-DDR2 antibody [HL1107] used in Western Blot (WB). GTX636322

GTX636322 WB Image

KO/KD

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (GTX636322) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDR2 antibody [HL1107] used in Western Blot (WB). GTX636322

GTX636322 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (GTX636322) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDR2 antibody [HL1107] used in IHC (Paraffin sections) (IHC-P). GTX636322

GTX636322 IHC-P Image

DDR2 antibody [HL1107] detects DDR2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
DDR2 stained by DDR2 antibody [HL1107] (GTX636322) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DDR2 antibody [HL1107] used in Western Blot (WB). GTX636322

GTX636322 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (GTX636322) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDR2 antibody [HL1107] used in IHC (Paraffin sections) (IHC-P). GTX636322

GTX636322 IHC-P Image

DDR2 antibody [HL1107] detects DDR2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
DDR2 stained by DDR2 antibody [HL1107] (GTX636322) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DDR2 antibody [HL1107] used in IHC (Paraffin sections) (IHC-P). GTX636322

GTX636322 IHC-P Image

DDR2 antibody [HL1107] detects DDR2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
DDR2 stained by DDR2 antibody [HL1107] (GTX636322) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DDR2 antibody [HL1107] used in Western Blot (WB). GTX636322

GTX636322 WB Image

U87-MG whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [HL1107] (GTX636322) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations