Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (20 ug of whole cell lysate)
A: human ESC
7.5% SDS PAGE
GTX110614 diluted at 1:2000

U87-MG whole cell and cytoplasm extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000.

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using DDX3(GTX110614) antibody at 1:200 dilution.

DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.