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DDX3 antibody

Anti-DDX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54030
Anti-DDX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54030
Anti-DDX3 antibody used in Western Blot (WB). GTX54030
Anti-DDX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54030
Anti-DDX3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54030
Anti-DDX3 antibody used in Immunoprecipitation (IP). GTX54030

Cat. No. GTX54030

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all DDX3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human DDX3X (NP_001347.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887835

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD-box helicase 3 X-linked , CAP-Rf , DBX , DDX14 , DDX3 , HLP2 , MRX102

Cellular Localization

Nucleus speckle,Cytoplasm,Mitochondrion outer membrane

Background

The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-DDX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54030

GTX54030 IHC-P Image

IHC-P analysis of human thyroid cancer tissue using GTX54030 DDX3 antibody.
Dilution : 1:100

Anti-DDX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54030

GTX54030 IHC-P Image

IHC-P analysis of rat brain tissue using GTX54030 DDX3 antibody.
Dilution : 1:100

Anti-DDX3 antibody used in Western Blot (WB). GTX54030

GTX54030 WB Image

WB analysis of various sample lysates using GTX54030 DDX3 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-DDX3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54030

GTX54030 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX54030 DDX3 antibody.
Dilution : 1:100

Anti-DDX3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54030

GTX54030 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54030 DDX3 antibody.
Blue : DAPI
Dilution : 1:100

Anti-DDX3 antibody used in Immunoprecipitation (IP). GTX54030

GTX54030 IP Image

IP analysis of C2C12 cell lysate using GTX54030 DDX3 antibody.
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

REFERENCE

Application Reference

Hueng DY et al. Int J Mol Sci 2015; 16 (7) : 15578-91 DDX3X Biomarker Correlates with Poor Survival in Human Gliomas.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399