Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DECR1 antibody (GTX109608) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX109608 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using DECR1(GTX109608) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (20 μg of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX109608 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DECR1 antibody [N1C3] detects DECR1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DECR1 protein stained by DECR1 antibody [N1C3] (GTX109608) diluted at 1:500.
Blue: Hoechst 33342 staining.