MCF-7 cytosol and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL2764] (GTX639629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (Cyto: cytosol extract; ME: membrane extract)

Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL2764] (GTX639629) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.