DHCR7 antibody [HL3685] detects DHCR7 protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: DHCR7 stained by DHCR7 antibody [HL3685] (GTX641713) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL3685] (GTX641713) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled MCF-7 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL3685] (GTX641713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)