Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody (GTX101245) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101245 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody (GTX101245) diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using DLD(GTX101245) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample(30 μg of whole cell lysate)
A:293T
10% SDS PAGE
GTX101245 diluted at 1:1000

DLD antibody detects DLD protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DLD protein stained by DLD antibody (GTX101245) diluted at 1:500.
Blue: Hoechst 33342 staining.