293T whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DLX2 antibody [HL1942] (GTX637779) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; NE: nuclear extract)

DLX2 antibody [HL1942] detects DLX2 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DLX2 stained by DLX2 antibody [HL1942] (GTX637779) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DLX2 antibody [HL1942] (GTX637779) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.