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DMP4 antibody [HL2677]

Recombinant antibody Orthogonal Overexpression
Anti-DMP4 antibody [HL2677] used in Western Blot (WB). GTX639341
Anti-DMP4 antibody [HL2677] used in Western Blot (WB). GTX639341
Anti-DMP4 antibody [HL2677] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639341

Cat. No. GTX639341

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2677

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DMP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Product Note

This antibody is specific for human FAM20C protein, and it does not cross react with human FAM20A and FAM20B protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human DMP4

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FAM20C golgi associated secretory pathway kinase , DMP-4 , DMP4 , G-CK , GEF-CK , RNS

Cellular Localization

Secreted,Golgi apparatus

Background

This gene encodes a member of the family of secreted protein kinases. The encoded protein binds calcium and phosphorylates proteins involved in bone mineralization. Mutations in this gene are associated with the autosomal recessive disorder Raine syndrome. [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Anti-DMP4 antibody [HL2677] used in Western Blot (WB). GTX639341

GTX639341 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with DMP4 antibody [HL2677] (GTX639341) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DMP4 antibody [HL2677] used in Western Blot (WB). GTX639341

GTX639341 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DMP4 antibody [HL2677] (GTX639341) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DMP4 antibody [HL2677] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639341

GTX639341 ICC/IF Image

DMP4 antibody [HL2677] detects DMP4 protein at cytoplasmic vesicles by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: DMP4 stained by DMP4 antibody [HL2677] (GTX639341) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations