Wild-type (WT) and POLB knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

DNA polymerase beta antibody [HL1512] detects DNA polymerase beta protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 10 min.
Green: DNA polymerase beta stained by DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

DNA polymerase beta antibody [HL1512] detects DNA polymerase beta protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
DNA polymerase beta stained by DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DNA polymerase beta antibody [HL1512] detects DNA polymerase beta protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
DNA polymerase beta stained by DNA polymerase beta antibody [HL1512] (GTX636986) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min