Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with DNA polymerase epsilon 3 antibody [N1C3] (GTX116557) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 ug of whole cell lysate)
A: 293T
15% SDS PAGE
GTX116557 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA polymerase epsilon(GTX116557) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DNA polymerase epsilon(GTX116557) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

DNA polymerase epsilon antibody [N1C3] detects DNA Polymerase epsilon protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
DNA Polymerase epsilon antibody [N1C3] (GTX116557) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min