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DNMT3B antibody

KO/KD Orthogonal Overexpression
Anti-DNMT3B antibody used in IHC (Whole mount) (IHC-Wm). GTX00735
Anti-DNMT3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00735
Anti-DNMT3B antibody used in Western Blot (WB). GTX00735
Anti-DNMT3B antibody used in Western Blot (WB). GTX00735

Cat. No. GTX00735

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IHC-Wm, IP, ChIP assay

Reactivity

 Mouse
Package
100 μg ($399)
See all DNMT3B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:3000
ICC/IF 1:5000
IHC-Fr 1:2000-1:5000
IHC-Wm Assay dependent
IP 1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Product Note

This antibody recognizes with mouse Dnmt3b isoforms 3b1, 3b2, 3b3 and 3b6, but does not react with Dnmt3a due to its immunogen.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant mouse Dnmt3b (amino acids 1-181)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA methyltransferase 3B , MmuIIIB

Cellular Localization

Nucleus

Background

This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene results in severe developmental defects and loss of viability. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. There is a pseudogene for this gene located adjacent to this gene in the same region of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]

Database

Research Area

DATA IMAGES

Anti-DNMT3B antibody used in IHC (Whole mount) (IHC-Wm). GTX00735

GTX00735 IHC-Wm Image

Orthogonal

IHC-Wm analysis of mouse embryos at E4.5, E5.5 and E7.0 using GTX00735 DNMT3B antibod (red). Dnmt3b existed at E4.5-7.0, in ICM at E4.5, the epiblast at E5.5, and the embryonic ectoderm at E7.0.
ICM : Inner Cell mass
T : Trophectoderm
E : Epiblast
EE : Embryonic Ectoderm
EX : Extraembryonic Region
Fixation : Cold Acetone

Anti-DNMT3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00735

GTX00735 ICC/IF Image

KO/KD

ICC/IF analysis of mouse embryonic stem cells using GTX00735 DNMT3B antibody.
Left : Dnmt3b in wild-type
Right : Dnmt3b/3a knockout
Dilution : 1:5000
Fixation : 4% PFA/PBS for 30 min at 4 degree C

Anti-DNMT3B antibody used in Western Blot (WB). GTX00735

GTX00735 WB Image

Orthogonal Overexpression

WB analysis of mouse embryos using GTX00735 DNMT3B antibody. Dnmt3b was highly expressed in E7.0 embryo but decreased thereafter and was below the detection level after E10.5.
Lane 1-7 : different stages within E7.0-E16.5 of mouse embryo (10 μg)
Lane 8-10 : 293T cells overexpressing 3b1, 3b2, and 3b3 of Dnmt3b isoforms

Anti-DNMT3B antibody used in Western Blot (WB). GTX00735

GTX00735 WB Image

KO/KD

WB analysis of mouse embryonic stem cells using GTX00735 DNMT3B antibody.
Left : mouse embryonic stem cells with wild-type Dnmt3b
Right : mouse embryonic stem cells with Dnmt3b/3a knockout
Dilution : 1:3000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399