WB analysis of mouse embryonic stem cells using GTX00735 DNMT3B antibody.
Left : mouse embryonic stem cells with wild-type Dnmt3b
Right : mouse embryonic stem cells with Dnmt3b/3a knockout
Dilution : 1:3000

WB analysis of mouse embryos using GTX00735 DNMT3B antibody. Dnmt3b was highly expressed in E7.0 embryo but decreased thereafter and was below the detection level after E10.5.
Lane 1-7 : different stages within E7.0-E16.5 of mouse embryo (10 μg)
Lane 8-10 : 293T cells overexpressing 3b1, 3b2, and 3b3 of Dnmt3b isoforms

ICC/IF analysis of mouse embryonic stem cells using GTX00735 DNMT3B antibody.
Left : Dnmt3b in wild-type
Right : Dnmt3b/3a knockout
Dilution : 1:5000
Fixation : 4% PFA/PBS for 30 min at 4 degree C

IHC-Wm analysis of mouse embryos at E4.5, E5.5 and E7.0 using GTX00735 DNMT3B antibod (red). Dnmt3b existed at E4.5-7.0, in ICM at E4.5, the epiblast at E5.5, and the embryonic ectoderm at E7.0.
ICM : Inner Cell mass
T : Trophectoderm
E : Epiblast
EE : Embryonic Ectoderm
EX : Extraembryonic Region
Fixation : Cold Acetone