APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain
Predict Reactivity
Human, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human DOC2A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10617987
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
double C2 domain alpha , Doc2
Cellular Localization
Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Peripheral membrane protein , Cell junction , synapse , synaptosome , Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane , Cell junction , synapse , synaptosome
Background
There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX119085 ICC/IF Image
DOC2A antibody [N2C3] detects DOC2A protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DOC2A stained by DOC2A antibody [N2C3] (GTX119085) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX119085 WB Image
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX119085 diluted at 1:1000
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REFERENCE
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REVIEW
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