Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DOCK1 antibody (GTX100243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human lung cancer, using DOCK 180 (GTX100243) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample(30 μg of whole cell lysate)
A:293T
7.5% SDS PAGE
GTX100243 diluted at 1:1000

Immunofluorescence analysis of methanol-fixed A431, using DOCK1/180(GTX100243) antibody at 1:200 dilution.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DOCK1 antibody (GTX100243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.