Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with DUSP1 antibody [N3C3] (GTX101229) diluted at 1:1000 and competitor's antibody (sc-1199) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.