Non-infected (–) and infected (+) BHK-21 whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus Envelope protein antibody (GTX127277) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody (GTX127277) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Envelope protein (Dengue virus) antibody detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody (GTX127277) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Envelope protein (Dengue virus) antibody detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody (GTX127277) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infected BHK-21
C: JEV infected BHK-21
12% SDS PAGE
GTX127277 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody (GTX127277) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein at cytoplasm by immunohistochemical analysis.
Sample: BHK-21 cells mock (left) and infected with Dengue virus (right) FFPE Cell Pellet Block.
Green: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody (GTX127277) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25412261