Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX124250) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX124250) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
10% SDS PAGE
GTX124250 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS4B (Dengue virus 2)(GTX124250) antibody at 1:2000 dilution.

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000.
Blue: Hoechst 33342 staining.

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25412261

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25412261

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25412261