Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX103557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Desmin antibody detects DES protein by Western blot analysis.
A. 30 μg A549 whole cell lysate/extract | B. 30 μg HCT116 whole cell lysate/extract
B. 30 μg HCT116 whole cell lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:3000

Desmin antibody detects DES protein by Western blot analysis.
A. 50 μg mouse muscle lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:10000

Desmin antibody detects Desmin protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
Desmin antibody (GTX103557) dilution: 1:1000

Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Desmin antibody detects DES protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
Desmin antibody (GTX103557) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Desmin antibody detects Desmin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Desmin antibody detects Desmin protein at cytosol on mouse skeletal muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skeletal muscle.
Desmin antibody (GTX103557) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX103557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Desmin antibody detects Desmin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog malignant soft tissue sarcoma.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Desmin antibody (GTX103557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Desmin antibody detects Desmin protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.

Desmin antibody detects Desmin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
Desmin stained by Desmin antibody (GTX103557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the journal PLoS One in 2016.PMID: 27589391