Whole cell extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Dishevelled 2 antibody [N2C2], Internal (GTX103878) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded human lung SCC, using Dishevelled 2(GTX103878) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample(30 μg whole cell lysate)
A: MOLT4 (GTX27912)
B: Raji (GTX27908)
7.5% SDS PAGE
GTX103878 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

cytoplasm in the photoreceptor
Immunohistochemistry of flat-mounted zebrafish retina using an antibody against Dishevelled 2 antibody [N2C2], Internal (GTX103878) at a 1:200 dilution.

The data was published in the 2020 in Front Cell Dev Biol. PMID: 33015048

The data was published in the journal PLoS One in 2013. PMID: 23437169