Sample (30 ug of whole cell lysate)
A: HCT116 7.5% SDS PAGE
GTX102509 diluted at 1:10000

Dishevelled 3 antibody [N1N2], N-term detects Dishevelled 3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dishevelled 3 protein stained by Dishevelled 3 antibody [N1N2], N-term (GTX102509) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Dishevelled 3 antibody [N1N2], N-term detects Dishevelled 3 protein at cytoplasm on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Dishevelled 3 antibody [N1N2], N-term (GTX102509) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

cytoplasm in the photoreceptor
Immunohistochemistry of flat-mounted zebrafish retina using an antibody against Dishevelled 3 antibody [N1N2], N-term (GTX102509) at a 1:200 dilution.

The data was published in the journal PLoS One in 2013. PMID: 23437169