Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D1 antibody [HL2680] (GTX639344) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.