Immunohistochemical analysis of paraffin-embedded human lung cancer, using EB3(GTX115652) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EB3 antibody (GTX115652) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.