EDC4 antibody detects EDC4 protein at P-body by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EDC4 protein stained by EDC4 antibody (GTX115745) diluted at 1:500.
Blue: Hoechst 33343 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

EDC4 antibody detects EDC4 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
EDC4 antibody (GTX115745) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 ug of whole cell lysate)
A: mouse brain
5% SDS PAGE
GTX115745 diluted at 1:2000

Sample (30 ug of whole cell lysate)
A: MCF-7
5% SDS PAGE
GTX115745 diluted at 1:3000

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using EDC4(GTX115745) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min