EFEMP1 antibody [HL2572] detects EFEMP1 protein by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EFEMP1 stained by EFEMP1 antibody [HL2572] (GTX638950) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EFEMP1 antibody [HL2572] (GTX638950) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

EFEMP1 antibody [HL2572] detects secreted EFEMP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
EFEMP1 stained by EFEMP1 antibody [HL2572] (GTX638950) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EFEMP1 antibody [HL2572] (GTX638950) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.