Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EFEMP1 antibody (GTX111657) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EFEMP1 antibody (GTX111657) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Fibulin 3(GTX111657) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EFEMP1 antibody (GTX111657) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Cancers (Basel) in 2019. PMID: 30754729

The data was published in the journal Cancers (Basel) in 2019. PMID: 30754729