Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
EGFR antibody [C2C3], C-term (GTX100448) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EGFR antibody [C2C3], C-term detects EGFR protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: EGFR stained by EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

EGFR antibody [C2C3], C-term immunoprecipitates EGFR protein in IP experiments.
IP samples: A431 whole cell extract
A. 40 μg A431 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of EGFR protein by 4 μg EGFR antibody [C2C3], C-term (GTX100448)
5 % SDS-PAGE
The immunoprecipitated EGFR protein was detected by EGFR antibody [C2C3], C-term (GTX100448) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer .
EGFR antibody [C2C3], C-term (GTX100448) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the 2017 in Oxid Med Cell Longev. PMID: 28740570