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EGFR antibody [GT461]

KO/KD Orthogonal IP/MS
Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888
Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888
Anti-EGFR antibody [GT461] used in Immunoprecipitation (IP). GTX628888
Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888
Anti-EGFR antibody [GT461] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628888
Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

Cat. No. GTX628888

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT461

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EGFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Observed MW

180 kDa.

Positive Control

A431 , A549

Product Note

This antibody was raised against human EGFR Intracellular domain.
IP/MS validation was supported by references (PMID:30377424)

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human EGFR. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888065

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

GTX628888 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:8000.

Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

GTX628888 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-EGFR antibody [GT461] used in Immunoprecipitation (IP). GTX628888

GTX628888 IP Image

Immunoprecipitation of EGFR protein from A431 whole cell extracts using 5 μg of EGFR antibody [GT461] (GTX628888).
Western blot analysis was performed using EGFR antibody [GT461] (GTX628888).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

GTX628888 WB Image

EGFR antibody [GT461] detects EGFR protein by Western blot analysis.
A. 30 μg A431 whole cell lysate/extract
5 % SDS-PAGE
EGFR antibody [GT461] (GTX628888) dilution: 1:10000

Anti-EGFR antibody [GT461] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628888

GTX628888 ICC/IF Image

EGFR antibody [GT461] detects EGFR protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde for 10 min.
Green: EGFR protein stained by EGFR antibody [GT461] (GTX628888) diluted at 1:300.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-EGFR antibody [GT461] used in Western Blot (WB). GTX628888

GTX628888 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zih-Yin Lai et al. Int J Mol Sci 2021; 22 (16) Gain-of-Function Mutant TP53 R248Q Overexpressed in Epithelial Ovarian Carcinoma Alters AKT-Dependent Regulation of Intercellular Trafficking in Responses to EGFR/MDM2 Inhibitor.
Application : ICC/IF
Reactivity : Human
Lai Z.Y. et al. Int. J. Mol. Sci. 2021; 22 (16) : 8784 Gain-of-Function Mutant TP53 R248Q Overexpressed in Epithelial Ovarian Carcinoma Alters AKT-Dependent Regulation of Intercellular Trafficking in Responses to EGFR/MDM2 Inhibitor.
Application : WB, ICC/IF
Reactivity : Human
Boyle J et al. Endocrine 2019; (Epub) CRH stimulation improves 18F-FDG-PET detection of pituitary adenomas in Cushing's disease.
Application : IHC-P
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations