Non-transfected (–) and transfected (+) A431 whole cell extracts (15 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:8000.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

EGFR antibody [GT461] detects EGFR protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde for 10 min.
Green: EGFR protein stained by EGFR antibody [GT461] (GTX628888) diluted at 1:300.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

EGFR antibody [GT461] detects EGFR protein by Western blot analysis.
A. 30 μg A431 whole cell lysate/extract
5 % SDS-PAGE
EGFR antibody [GT461] (GTX628888) dilution: 1:10000

Immunoprecipitation of EGFR protein from A431 whole cell extracts using 5 μg of EGFR antibody [GT461] (GTX628888).
Western blot analysis was performed using EGFR antibody [GT461] (GTX628888).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.