Immunofluorescence analysis of methanol-fixed A431, using EIF3A(GTX118895) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded human hepatoma, using EIF3A(GTX118895) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

EIF3A antibody [N1N2], N-term detects EIF3A protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF3A protein stained by EIF3A antibody [N1N2], N-term (GTX118895) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Sample (30 ug of whole cell lysate)
A: Raji
5% SDS PAGE
GTX118895 diluted at 1:500