Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ELAVL1 / HuR antibody [GT1182] (GTX00944) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ICC/IF analysis of PFA-fixed C6 cells using GTX00944 ELAVL1 / HuR antibody [GT1182].
Orange : Primary antibody
Blue : DAPI

ICC/IF analysis of PFA-fixed U2OS cells using GTX00944 ELAVL1 / HuR antibody [GT1182].
Orange : Primary antibody
Blue : DAPI

IHC-P analysis of rat liver tissue section using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:100

WB analysis of various samples using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:1000
Loading : 25μg

IHC-P analysis of human esophageal tissue section using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:100

IP analysis of 293T cell lysate using GTX00944 ELAVL1 / HuR antibody [GT1182].
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00944 ELAVL1 / HuR antibody [GT1182].
Orange : Primary antibody
Blue : DAPI

IHC-P analysis of mouse spleen tissue section using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:100

WB analysis of wild-type (WT) and ELAVL1 / HuR knockout (KO) 293T cell extracts using GTX00944 ELAVL1 / HuR antibody [GT1182].
Dilution : 1:1000
Laoding : 25μg