APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , ELMO2 transfected 293T cell , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ELMO2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950194
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
engulfment and cell motility 2 , CED-12 , CED12 , Ced-12A , ELMO-2 , VMPI
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein. Similarity to a C. elegans protein suggests that this protein may function in phagocytosis of apoptotic cells and in cell migration. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX108070 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELMO2 antibody [C3], C-term (GTX108070) diluted at 1:1000.
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GTX108070 WB Image
Western Blot analysis of ELMO2 expression in transfected 293T cell line by ELMO2 polyclonal antibody. A: Non-transfected lysate. B: ELMO2 transfected lysate. 12% SDS PAGE GTX108070 diluted at 1:500
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GTX108070 WB Image
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX108070 diluted at 1:1000
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GTX108070 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELMO2 antibody [C3], C-term (GTX108070) diluted at 1:500.
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REFERENCE
There are currently no references for ELMO2 antibody [C3], C-term (GTX108070). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ELMO2 antibody [C3], C-term (GTX108070). Be the first to share your experience with this product.