Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
EML1 antibody
GTX100252 diluted at 1:500

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX100252 diluted at 1:1000

EML1 antibody [C3], C-term detects EML1 protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
EML1 antibody [C3], C-term (GTX100252) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

EML1 antibody [C3], C-term detects EML1 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
EML1 antibody [C3], C-term (GTX100252) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

EML1 antibody [c3], c-term detects EML1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EML1 protein stained by EML1 antibody [c3], c-term (GTX100252) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded RF48 xenograft, using EML1(GTX100252) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min