Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human gastric tissue, using ENO1(GTX101803) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
B: H1299
10% SDS PAGE
GTX101803 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101803 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ENO1 antibody [N3C3] detects ENO1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ENO1 protein stained by ENO1 antibody [N3C3] (GTX101803) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX101803 diluted at 1:1000

ENO1 antibody [N3C3] detects Eno1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
ENO1 antibody [N3C3] (GTX101803) dilution: 1:100.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ENO1 antibody [N3C3] (GTX101803) at 1:300 dilution.

The data was published in the journal BMC Genomics in 2015. PMID: 25652794