ENO1antibody immunoprecipitates ENO1protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of ENO1protein by 2.5 μg of ENO1 antibody (GTX113179) 10% SDS-PAGE The immunoprecipitated ENO1 protein was detected by ENO1 antibody (GTX113179) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody (GTX113179) diluted at 1:20000.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody (GTX113179) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HCT116, using ENO1(GTX113179) antibody at 1:500 dilution.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody (GTX113179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody (GTX113179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ENO1(GTX113179) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal Viruses in 2018. PMID: 29518929