Wild-type (WT) and ENSA knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ENSA antibody (GTX101493) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ENSA antibody detects ENSA protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold Methanol for 5 min.
Green: ENSA protein stained by ENSA antibody (GTX101493) diluted at 1:500.
Blue: Hoechst 33343 staining.

ENSA antibody detects ENSA protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
ENSA antibody (GTX101493) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ENSA antibody detects ENSA protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
ENSA antibody (GTX101493) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ENSA antibody detects ENSA protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ENSA antibody (GTX101493) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min