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ENaC Alpha antibody [HL2967]

Recombinant antibody Orthogonal Overexpression
Anti-ENaC Alpha antibody [HL2967] used in Western Blot (WB). GTX640354
Anti-ENaC Alpha antibody [HL2967] used in Western Blot (WB). GTX640354

Cat. No. GTX640354

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2967

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ENaC Alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Product Note

This antibody was raised against human ENaC Alpha Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment encompassing a sequence within the Extracellular domain of human ENaC Alpha. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sodium channel epithelial 1 alpha subunit , BESC2 , ENaCa , ENaCalpha , LIDLS3 , SCNEA , SCNN1

Cellular Localization

Apical cell membrane,Cell projection, cilium,Cytoplasm,Cell projection, cilium, flagellum

Background

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-ENaC Alpha antibody [HL2967] used in Western Blot (WB). GTX640354

GTX640354 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ENaC Alpha antibody [HL2967] (GTX640354) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ENaC Alpha antibody [HL2967] used in Western Blot (WB). GTX640354

GTX640354 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ENaC Alpha antibody [HL2967] (GTX640354) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Custom Conjugations